The Shakespeare play that features the characters of Othello and Desdemona is called "Othello." This tragic play, written around 1603, explores themes of jealousy, betrayal, love, and racism. Othello, a Moorish general in the Venetian army, falls in love with Desdemona, but their marriage faces turmoil due to the manipulations of Iago, Othello's envious ensign. Iago's deceit leads Othello to doubt Desdemona's fidelity, resulting in tragic consequences. The play is renowned for its exploration of human emotions and moral complexity, making it a significant work in the realm of English literature.