Sitting for prolonged periods has a significant negative impact on overall metabolic function. Extended sedentary behavior is associated with decreased energy expenditure, leading to weight gain and increased risk of chronic conditions like obesity, heart disease, and type 2 diabetes. It also negatively affects muscle metabolism and insulin sensitivity by limiting physical activity, which plays a crucial role in maintaining metabolic health. To counteract these effects, incorporating regular breaks for movement, such as standing or walking, is essential to promote better metabolic outcomes and overall health.