The main cause of dissent during the reign of King George III in the American colonies was the imposition of various taxes and regulations without representation in Parliament. Acts such as the Stamp Act and the Tea Act angered colonists, who felt their rights as Englishmen were being violated. The growing sentiment of "no taxation without representation" fueled a desire for independence and self-governance. Additionally, the increasing control exerted by the British government stirred resentment among colonists who valued their autonomy. This dissent ultimately culminated in the American Revolution, as colonists sought to break free from British rule and establish their own nation.